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Representative <t>2D-DIGE</t> gel image of differentially expressed proteins of fetal ovaries at day 55 and day 90 of gestation. The proteins extracted from the fetal ovaries at day 55 and day 90 of gestation samples were labelled with cy3 and cy5, respectively. An internal standard protein sample (a mixture of fetal ovaries at day 55 and day 90 of gestation samples) was labelled with the Cy2 dye. The number in the figure corresponds to the number shown in .
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Image Search Results


Representative 2D-DIGE gel image of differentially expressed proteins of fetal ovaries at day 55 and day 90 of gestation. The proteins extracted from the fetal ovaries at day 55 and day 90 of gestation samples were labelled with cy3 and cy5, respectively. An internal standard protein sample (a mixture of fetal ovaries at day 55 and day 90 of gestation samples) was labelled with the Cy2 dye. The number in the figure corresponds to the number shown in .

Journal: BioMed Research International

Article Title: Proteomic Analysis of Fetal Ovaries Reveals That Primordial Follicle Formation and Transition Are Differentially Regulated

doi: 10.1155/2017/6972030

Figure Lengend Snippet: Representative 2D-DIGE gel image of differentially expressed proteins of fetal ovaries at day 55 and day 90 of gestation. The proteins extracted from the fetal ovaries at day 55 and day 90 of gestation samples were labelled with cy3 and cy5, respectively. An internal standard protein sample (a mixture of fetal ovaries at day 55 and day 90 of gestation samples) was labelled with the Cy2 dye. The number in the figure corresponds to the number shown in .

Article Snippet: The 2D DIGE gel images were analyzed by the Image Master 2D platinum 7.0 software (GE Healthcare Life Sciences, NJ, USA) and the protein abundance changes for spot picking detection were calculated using cy3/cy2 and cy5/cy2 differential in-gel analysis ratios.

Techniques:

2-DE pattern (the master gel from nine gels) of germinated Pt race-1 urediniospores on 0.5% agarose for 24 h at 20 °C. The image was created by Proteomescan software. Gel dimensions: 24 × 20 cm. Proteins were separated with a pH 4–7 gradient in the 1st dimension, and on a 10–20% gradient SDS-PAGE in the 2nd dimension.

Journal: Scientific Reports

Article Title: The proteome study of germinated Puccinia triticina urediniospores reveals a novel effector protein required for virulence

doi: 10.1038/s41598-026-44996-2

Figure Lengend Snippet: 2-DE pattern (the master gel from nine gels) of germinated Pt race-1 urediniospores on 0.5% agarose for 24 h at 20 °C. The image was created by Proteomescan software. Gel dimensions: 24 × 20 cm. Proteins were separated with a pH 4–7 gradient in the 1st dimension, and on a 10–20% gradient SDS-PAGE in the 2nd dimension.

Article Snippet: Gels were stained with colloidal coomassie brilliant blue (CBB) and digitalized by Syngene Proteomescan Pro 2D Gel Imaging Systems at 800 dpi, and Dymension (Syngene) was used for image analysis.

Techniques: Software, SDS Page